Reporter

Part:BBa_K1033928:Design

Designed by: Sabri Jamal   Group: iGEM13_Uppsala   (2013-09-17)


aeBlue, blue chromoprotein (incl RBS, J23110)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthesized and codon optimized for E. coli by Bioneer Corp.


Source

Actinia equina

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/] Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.